FastQCFastQC Report
Wed 25 May 2016
SRR1699968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5666637
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN233540.4121315693946868No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN111410.19660691164794922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN84230.14864195465493907No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN74830.1320536325160761No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN69240.12218887498881612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN64630.11405353828028864No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN61230.10805350686835949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG61650.025.2971941
TACCTGG62300.024.4360162
ACCTGGG67100.022.1630133
GTACCCG7100.017.9945851
TAGACCG750.001292027117.599275
CCTGGGG50650.017.3734154
TACCCGG7100.017.3516982
ACGATCG1401.5890437E-615.71391120
GTATAAC10950.015.087571
TATAACG4150.014.84301952
TAACGCA4450.014.8308464
GTACCGG3900.014.6852351
GTACACG5100.014.6852351
GACGCTA3450.014.66657540
GTCTAGG9250.013.5739191
ACGCTAC3850.013.14277541
TACACGG5050.013.06899452
GTACATG149900.012.8287631
TATCACG4300.012.7903922
CGACCGT1902.6584203E-612.73642521
Produced by FastQC (version 0.11.5)