FastQCFastQC Report
Wed 25 May 2016
SRR1699967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5821679
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN356960.6131564450736635No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN179070.3075916758722011No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN118280.20317162797880128No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN112520.19327757507756782No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN91520.15720550720848744No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN78470.13478929360413036No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN74410.1278153604827748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN69390.11919241854454701No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCN67620.11615205853843882No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGN60460.10385320111259999No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG62250.025.798222
GTACCTG63850.025.565441
ACCTGGG68400.023.060533
TATAACG3800.019.104542
ATAACGC4050.018.4684353
CCTGGGG52100.018.4100514
TAACGCA3250.018.2762324
CGACCGT1355.5370037E-817.92570921
GTACCGG5100.015.9818691
GTACACG6350.015.9580591
TACCCGG5600.015.7136922
GTACCCG6400.015.4891821
GTATTAG16050.014.2742821
GTCGTAC1554.511443E-614.2122971
GTATAAC11350.013.9743821
CGCGTAT950.006244464313.8944512
CGATCGT2703.274181E-1113.85132741
TACACGG6150.013.5929812
GAGTACG6350.013.5296591
GTACATG140950.013.42530251
Produced by FastQC (version 0.11.5)