FastQCFastQC Report
Wed 25 May 2016
SRR1699966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7254528
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN85790.11825717675912203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN76630.10563057996330015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG2650.019.9240120
CGCGAAA2700.019.55504625
TACCTGG51900.018.820322
GTACCTG55000.018.5449541
ATCGCGA3000.016.86622623
CGCGCAA5450.016.54991318
TCGCGAA3250.016.24573124
ACCTGGG59250.016.0400893
GTATCAA181200.015.5374631
TATACTG22000.015.09950355
GTATTAG17500.014.8542741
TACCGTC3000.014.6661837
TCGCGCA6050.014.54497517
AATCGCG5900.014.54189415
CGCAACG6100.014.42585420
GGTATCA117200.014.3982131
AACGATA6700.014.11903523
GTACATG184700.013.9906721
GTATAGG15400.013.4466651
AGCGTCG3800.013.31535140
Produced by FastQC (version 0.11.5)