FastQCFastQC Report
Wed 25 May 2016
SRR1699965_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699965_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6415455
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN98410.15339519956105999No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN98180.15303669030489653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN68920.10742807797732194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG90600.027.4006791
TACCTGG89450.027.2745742
ACCTGGG93500.025.5284753
GTACACG6000.021.2932681
CCTGGGG71900.021.050694
TATAACG4600.021.0427072
ATAACGC4950.018.6659833
GTACCCG7950.017.7328451
TAACGCA5600.017.6779254
TACCCGG8100.017.1104832
CTGGGGG37900.016.6590215
CCGTCGA800.001990096416.49939541
TACACGG6300.016.4120962
ACGATCG1259.455463E-615.83954120
GTACCGG7000.015.1045861
CGCGAAA1351.9591085E-514.66624425
GTATCAA180350.014.6320871
GGTATCA119350.014.1190211
TTTCGCG3001.8189894E-1213.93282136
CGATCGT3001.8189894E-1213.93282141
Produced by FastQC (version 0.11.5)