FastQCFastQC Report
Wed 25 May 2016
SRR1699964_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699964_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5305412
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN129380.24386419000070117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN101500.191314077021728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN73350.1382550497491995No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN59050.11130144086830579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG52800.023.792382
GTACCTG54900.023.5576671
ACCTGGG56250.021.744793
ACGATCG1408.3868144E-817.28590820
TAACGCA3450.017.216614
CGCGAAA1551.4430043E-817.03148525
ATAACGC3900.016.9223083
GTACACG4400.016.5244791
CGCGTAT1105.6291916E-515.99927312
CCTGGGG45800.015.7547434
GTACCGG5250.015.5277641
TACACGG4400.015.5004642
TACCCGG5250.015.0861672
TATAACG4400.015.000452
TCGCGCA3200.014.4376617
GTACCCG6000.014.3212161
CGCGCAA3450.014.02914118
GTATCAA187100.013.9897251
ATCGCGA2052.9351213E-813.95058623
CGCAACG3350.013.79119920
Produced by FastQC (version 0.11.5)