FastQCFastQC Report
Wed 25 May 2016
SRR1699963_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699963_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6130722
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN212590.34676176802666964No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN103100.168169426048025No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN71680.11691934489934463No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN69900.11401593482790445No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN68210.11125932638928987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG53900.022.5233651
TACCTGG53900.021.8361532
TAACGCA3600.020.1656364
ACCTGGG57900.019.7951893
ATAACGC4300.017.9060613
CGCGCAA3200.017.18830318
TATAACG4500.016.6217772
GTACCCG5750.014.9440111
CCTGGGG49050.014.4865164
AACGATA3650.014.4652523
ACGATCG1402.7592456E-514.14340320
ATCGCGC3950.013.92447416
AATCGCG4000.013.74940915
TCGCGCA3850.013.71492717
CGATCGT2754.3655746E-1113.59930341
GTATAAC12400.013.5039971
GCGCAAC4200.013.09574419
TACCCGG6250.013.0236532
GTACATG145650.012.9489241
CGACGCT3153.092282E-1112.57191421
Produced by FastQC (version 0.11.5)