FastQCFastQC Report
Wed 25 May 2016
SRR1699962_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699962_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4932244
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN92090.18671014653776252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN64720.13121816357828203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG69950.027.8422471
TACCTGG69600.027.563512
ACCTGGG71150.026.2822483
TAACGCA3800.024.8934864
CCTGGGG52700.022.3745864
TATAACG4650.022.2367952
ATAACGC4450.021.75263
GGTATCA59000.020.9110431
GTATCAA109250.020.1860961
GTACACG5100.019.871351
TACACGG5100.018.9805952
GTATAAC10400.017.7944951
CTGGGGG27150.017.2588025
GTACCCG5600.016.9168761
GTACCGG4950.016.0227491
ATCAACG134250.015.8955633
TCAACGC135200.015.8157734
TATCAAC139550.015.4498222
CAACGCA139550.015.322775
ACACGGG6500.015.2306223
Produced by FastQC (version 0.11.5)