FastQCFastQC Report
Wed 25 May 2016
SRR1699961_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699961_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6298098
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN262790.4172529547809513No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN134260.2131754698005652No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN95840.15217292585793363No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN90010.14291616294316156No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN71290.1131929036353515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG51900.019.7114832
GTACCTG54750.019.0776581
ACCTGGG58100.017.1143953
GTACACG5500.015.2248051
TACACGG4850.014.9694522
GTATTAA16900.014.0821141
GTACATG191300.013.7191621
GTATTAG16100.013.6868941
AACGATA6600.013.66603823
TCGCGCA6750.013.3634117
TACATGG189550.013.27812
GTATAGA18150.012.9908621
ATCGCGC6800.012.94150516
CGCGCAA6650.012.90268618
AATCGCG6500.012.86084415
GCGCGTT4850.012.70034531
ACATGGG193650.012.632423
GCAACGA7400.012.48690421
CCTGGGG53500.012.3769044
TATGCCG7200.012.22156243
Produced by FastQC (version 0.11.5)