FastQCFastQC Report
Wed 25 May 2016
SRR1699960_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699960_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3681558
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN152360.4138465291053407No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN69060.1875836262799608No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN55980.15205518967784834No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN49350.13404650965705278No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN45130.12258397124260978No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN40150.10905708941703486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN37790.10264675987720416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG53350.029.8851241
TACCTGG54450.028.8079092
ACCTGGG56150.027.3088343
TATAACG3550.026.647732
ATAACGC3850.024.5712853
TAACGCA3850.023.9992074
CCTGGGG41250.021.7592814
GTACACG3200.020.6453481
CGCGAAA550.00448618119.9993425
ATCGCGA550.00448618119.9993423
GTACCGG3650.018.7033651
TACCCGG4000.018.699892
CGACCGT1303.594505E-818.61476921
GTACCCG4550.017.907761
GTATAAC8250.017.8842931
CGCGCAA1053.7770376E-516.7622618
ACGTCGC953.349483E-416.20999122
CTGGGGG22200.015.9544285
ACACGGG3650.015.6711413
TACACGG3700.015.4593682
Produced by FastQC (version 0.11.5)