FastQCFastQC Report
Wed 25 May 2016
SRR1699959_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699959_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6786974
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN92710.13659990446405126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70950.022.6631531
GTATCAA134900.020.9000761
GTACCTG51200.018.8861941
TACCTGG52450.018.1195342
CGCGCAA4750.017.13730218
TAACGCA4050.016.8388444
ATCAACG167100.016.6278363
TCAACGC167900.016.5091784
ACCTGGG56200.016.323323
ACGAACG2450.016.16262829
CAACGCA174050.015.938475
AATCGCG5150.015.80625115
TATCAAC178150.015.6582152
GCGCAAC5250.015.50517919
AACGCAG181150.015.3866436
CGCAACG5300.014.94280820
CGCGAAA2002.1218511E-814.299437525
ACGATCG2002.1218511E-814.299437520
TCGCGCA5750.014.15690417
GTACATG180100.013.5022211
Produced by FastQC (version 0.11.5)