FastQCFastQC Report
Wed 25 May 2016
SRR1699958_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699958_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7095610
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN93260.13143337923025644No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN73710.10388113213663096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG62050.023.0752351
TACCTGG63650.022.1549452
ACCTGGG69500.019.6566123
GGTATCA89900.019.332671
GTATCAA154750.017.7931941
GTACCGG5300.017.456131
AATCGCG4550.014.98924415
CCTGGGG52900.014.9293414
ATCAACG182550.014.7141683
ATCGCGA2254.292815E-1014.66617123
TCAACGC183600.014.6180354
ATAACGC5900.014.5416773
TAACGCA4850.014.5147694
CAACGCA189300.014.1894955
TATCAAC190450.014.173422
GTATTAG18700.014.1355971
TATAACG5600.013.7496322
AACGCAG196650.013.7150816
CGCGAAA2256.7393557E-913.68842625
CGCGCAA4950.013.3335418
Produced by FastQC (version 0.11.5)