FastQCFastQC Report
Wed 25 May 2016
SRR1699957_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699957_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4707277
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN144950.30792749183869994No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN69590.14783493726840377No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN68200.14488206238978502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN55840.11862484404465681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2550.022.431142
GTACCTG46100.021.9301851
TACCTGG46150.021.4038942
ACGATCG1502.1827873E-1120.53355620
ACCTGGG50350.018.9624563
ACGACCG1154.2799165E-617.2166635
TAGCCCG1307.3311094E-716.9223635
ATCGCGA1906.184564E-1016.21001423
ATAACGC3850.015.9993243
TAACGCA3500.015.0850764
TACACGG3550.014.8730842
TATACTG14650.014.7161025
CGCGCAA3350.014.44776418
GTACCCG3050.014.4431091
AATCGCG3400.014.235146515
CCTGGGG40250.013.9919564
TCGCGCA3550.013.63395117
GTATAAC9000.013.4601761
GTATTAA14450.013.1087471
CGACCGT1353.2100486E-413.03717921
Produced by FastQC (version 0.11.5)