FastQCFastQC Report
Wed 25 May 2016
SRR1699956_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699956_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8319149
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN265700.3193836292630412No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN141590.170197696903854No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN93490.11237928302522289No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN86530.10401304268020684No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN83560.10044296598125602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAA3700.019.02676424
ACGATCG4200.016.23787120
ATCGCGA4200.016.23787123
CGCGAAA4200.016.23777425
TATACCG3150.016.0628835
GTACCTG55100.014.9127931
TACCTGG54050.014.774652
AATCGCG8850.014.66637515
CGCGCAA9300.014.19309618
AACGATA9900.013.99980523
AGCGTCG5200.013.9610140
TCGCGCA9300.013.95671317
GTCGGTA5050.013.94006543
CGATCGC5350.013.56990721
CGCAACG10100.013.50476520
GCGCAAC9950.013.26598919
GTACATG243050.013.1605121
ATACCGT7050.013.1058866
GATCGCG5450.012.91725222
GTATATA30250.012.817081
Produced by FastQC (version 0.11.5)