FastQCFastQC Report
Wed 25 May 2016
SRR1699955_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699955_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4787193
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN149810.31293912737589646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN71310.14895994374991775No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN70170.14657859000044493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN55100.11509876455785258No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN48490.10129109062450585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG56250.026.1533161
TACCTGG56450.025.5652352
ACCTGGG62250.023.0065523
TATAACG4150.021.2041952
TAACGCA4150.020.1439864
ATAACGC4450.019.77473
CCTGGGG47250.018.4375534
GTATAAC8650.018.076531
TACCCGG4500.017.5994822
TACACGG4300.016.371612
GTACACG4450.016.3315261
CTACGCT1351.0874246E-616.2958164
GTACCCG5100.015.9773351
CTGGGGG25650.014.83776955
GTCTCGC5800.014.0490371
TCACGCA4450.013.8422914
GTACCGG4350.013.6693331
TATCGGA1952.411125E-713.53820419
GTATTAA16350.013.0655231
GTACATG99200.012.9428431
Produced by FastQC (version 0.11.5)