FastQCFastQC Report
Wed 25 May 2016
SRR1699954_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699954_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7970657
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG57600.019.0582622
GTACCTG59750.018.913691
GTACACG6100.018.4177721
ACCTGGG64100.016.4392763
TACACGG5900.016.406122
GTATCAA177400.016.1927551
GGTATCA111900.016.0641171
CGCAACG6450.015.00743120
AATCGCG6400.014.78121215
ACGATCG2254.292815E-1014.66635220
CGCGCAA6750.014.34016418
TACACCG5150.013.66935355
GTACATG183150.013.4592221
ATCAACG209300.013.4222993
TCAACGC210950.013.3381694
TATACTG26800.013.2979735
TCGCGCA7300.013.2602417
CGCGATT3500.013.1994731
TACATGG182950.013.082832
ATCGCGA2704.7111826E-1013.036756523
Produced by FastQC (version 0.11.5)