FastQCFastQC Report
Wed 25 May 2016
SRR1699953_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699953_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5296704
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN85760.1619120116963304No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN85280.16100578775026883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN60660.11452405118352847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG67650.026.959532
GTACCTG70100.026.6865391
ACCTGGG70100.025.356793
CCTGGGG57450.020.1034344
GTACCGG5050.018.7620241
TAACGCA4050.017.925034
TATAACG3600.017.7223422
GTACACG4550.016.4653171
TACACGG4700.016.3830872
ATAACGC4300.015.8596723
CGCTTAT2700.015.48070724
CTGGGGG31200.015.3710265
GTATCAA151250.014.6847911
GTACCCG6150.014.3313631
TCTGTCG8200.014.2188018
GTATAAC9250.013.5779981
TAGACCG1302.3338322E-413.5377855
GACGCTA3101.8189894E-1213.48319740
CTGTCGC8650.013.4790959
GTATTAG13950.013.2680671
Produced by FastQC (version 0.11.5)