FastQCFastQC Report
Wed 25 May 2016
SRR1699952_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699952_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6262577
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN80910.12919601627253444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG58000.023.5171572
GTACCTG59150.023.506411
ACCTGGG65400.020.0478973
GTATCAA139100.017.1241991
CCTGGGG54100.015.8993654
GGTATCA86300.015.6516941
ATAACGC4050.015.2090933
TACACGG5050.014.3761852
ATCGCGA1402.761194E-514.14235423
TCAACGC168100.013.7934594
ATCAACG167800.013.79193
GTATAGG12700.013.7067681
TATACTG19650.013.6583295
GTACATG142550.013.6027641
GTACACG5550.013.4988781
GTATTAG17200.013.4515571
CAACGCA172500.013.4288735
TATCAAC174150.013.4159722
GTACCCG5750.013.4125671
TAACGCA3300.013.3326464
Produced by FastQC (version 0.11.5)