FastQCFastQC Report
Wed 25 May 2016
SRR1699951_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699951_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6829820
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN624440.9142847102851905No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN316960.4640825087630421No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN208470.3052349842309168No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN204820.29989077310968665No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN157970.23129452899197928No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN141420.20706255801763446No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN133240.19508566843635702No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCN119860.1754951082166148No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGN103680.1518048791915453No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAN97820.14322485804896762No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAN96120.14073577341716179No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN93090.13629934610282557No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCN88310.1293006257851598No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCN69940.10240387008735223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG56800.020.0899581
TACCTGG55800.019.9887662
ACCTGGG61450.017.6137683
CGATCGT4250.017.0814441
ATCGCGA2350.016.85016423
TCGCGAT4800.016.49911938
ACGATCG2450.016.16275620
ACCGACG4700.015.44779419
ATAACGC4050.015.2093983
TTTCGCG4950.015.11030436
CGCGAAA2651.8189894E-1214.94259825
TTCGCGA4900.014.81553537
CGACGCT4950.014.66599121
TACCGAC5200.014.38574218
GTCGGTA3100.014.1927943
CGCGCAA5400.013.85293818
CCTGGGG49950.013.52084
GTACATG149200.013.4655351
GTACACG4750.013.4493721
TACATGG147150.013.08157352
Produced by FastQC (version 0.11.5)