FastQCFastQC Report
Wed 25 May 2016
SRR1699950_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699950_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5490799
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN177360.32301309882222967No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN81700.148794374006406No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN81410.14826621772168314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN58430.10641438522881644No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN57380.10450209523240608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG74100.028.1308161
TACCTGG73900.027.7757512
ACCTGGG77150.025.9196343
CCTGGGG64200.020.7310494
GTACACG5500.019.6310181
TACACGG5950.018.1178952
TAACGCA4350.017.194544
ACGATCG1307.3280717E-716.92315520
TATAACG4700.016.8512922
GTACCGG5250.016.368721
ATAACGC5050.016.1180063
CTGGGGG31250.015.8392075
CGCGCAT1004.9488584E-415.39950812
CGCGAAA1603.8327562E-715.12424225
GTACCCG6200.014.926811
ACACGGG7550.014.5688073
AATCGCG3350.014.44730115
TACCCGG5800.014.4139892
ATCGCGA1554.575377E-614.19296723
CGCGCAA3350.013.79135918
Produced by FastQC (version 0.11.5)