FastQCFastQC Report
Wed 25 May 2016
SRR1699949_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699949_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4781097
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN273280.571584303769616No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN132350.2768193157344434No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN90970.19027014093209152No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN84590.17692592306744664No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN72500.15163883937096445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN71830.15023748733815692No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN61430.12848515727666684No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN54730.1144716369485915No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCN50650.10593803053985308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN50560.10574978922201328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG62450.027.8649232
GTACCTG63600.027.8512381
ACCTGGG66750.025.4436763
CCTGGGG51050.021.287834
TATAACG3900.020.3072782
TATCGCG550.004486862619.9989627
ATAACGC3650.019.8900053
GTACACG4850.019.0788921
TAACGCA4000.017.5990894
TACACGG5250.017.1806012
GTACCGG4950.016.913131
CGTTCGA1004.949441E-415.3992038
CTGGGGG29700.015.3325395
GTACCCG4950.015.13281
CGATCGT2102.5484042E-914.6674414
CGTGCGA1057.167113E-414.66590739
GCGCGTT2254.292815E-1014.66590631
CGTTCCG2305.966285E-1014.34708234
GTATAAC8650.014.0085171
TAGACTG9150.013.9446335
Produced by FastQC (version 0.11.5)