FastQCFastQC Report
Wed 25 May 2016
SRR1699948_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699948_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4003046
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN160750.4015692050503542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN93050.23244799085496395No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN75370.18828162354367148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN66400.16587368718720694No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN51700.12915165101774997No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN51670.12907670808679192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN46360.11581180930721256No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN40580.10137280460929003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG42550.026.058282
GTACCTG43950.026.015121
ACCTGGG45700.023.8770283
TAGACCG602.875223E-421.9990525
CCTGGGG36750.019.634534
TAACGCA3000.018.3325424
TATAACG3200.016.49972
ATAACGC3400.015.5291313
GTACACG3750.015.2742341
GTACCGG3050.015.1682981
CTGGGGG21450.014.25579555
GTACATG96600.013.8429591
TACACGG4100.013.4143912
GAGTACT101050.013.12775112
TACATGG96950.013.0704162
AGAGTAC147350.013.0038511
GTACCGA1557.08874E-512.7916971
GTATAGA12000.012.6673341
TAGACAG9600.012.6036235
AGTACTT106050.012.48868913
Produced by FastQC (version 0.11.5)