FastQCFastQC Report
Wed 25 May 2016
SRR1699947_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699947_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4687581
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN89060.18999138361555779No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN77690.16573580275199512No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN64790.13821627828937783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG49600.025.8855611
TACCTGG49600.025.325442
ACCTGGG54550.022.3014433
TATAACG3750.020.5323832
TAACGCA3650.020.49224
ATAACGC4050.019.554653
CCTGGGG39150.018.8241624
CGCGAAA1456.322807E-918.20644225
CGCGCAA2850.016.98239318
GTATCAA150450.015.1502181
GTACACG4250.015.027261
GCGCAAC3300.014.66692619
ATCGCGA1809.07221E-814.666323
CTAACGG900.00436283714.6659873
TACCCGG3650.014.4650832
ACGATCG1851.2677992E-714.27052320
GTACCGG5250.014.2623251
GTATAAC9000.014.192411
TCGCGCA3300.014.00024817
AATCGCG3300.014.00009915
Produced by FastQC (version 0.11.5)