FastQCFastQC Report
Wed 25 May 2016
SRR1699946_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699946_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5869233
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN94940.16175878517687065No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN75210.12814280843851317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG52350.022.9301911
TACCTGG51350.022.3634932
ACCTGGG58700.018.7384683
AATCGCG4250.018.11833415
TATAACG2850.016.9819432
CGCGCAA4850.015.87662118
CGCGTAT850.002982372715.52894312
GTATCAA162150.015.4851631
ACGATCG2455.456968E-1215.26562620
GCAATCG5700.014.66622413
ATCGCGA2407.2759576E-1114.66597423
GCGCAAC5350.014.39282319
AACGATA5700.013.8940823
CCTGGGG48950.013.8869854
TCGCGCA5800.013.6556917
GGTATCA105750.013.5590251
ATAACGC3900.013.5378223
CGATCGT4250.013.45853141
GCAACGA5800.013.27568621
CGCAACG6050.013.09118320
Produced by FastQC (version 0.11.5)