FastQCFastQC Report
Wed 25 May 2016
SRR1699945_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699945_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5610105
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN613331.0932593953232606No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN297010.5294196810933129No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN203790.36325523319082265No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN200130.3567312911255672No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN154140.274754215830185No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN137760.24555690134141878No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN137480.24505780194844837No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCN124580.22206357991517092No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGN112680.20085185571393047No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAN100630.1793727568378845No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCN93550.16675267218706247No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAN91650.16336592630619215No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCN70550.1257552220502112No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN60570.1079658936864818No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACN59600.10623687078940591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCA7600.021.13212217
AATCGCG7800.020.58953715
ATCGCGA2950.020.13485523
CGCAACG8050.019.67752520
CGCGCAA8250.019.46699318
GCGCAAC8650.018.56695219
ACGATCG3300.018.00046320
CGCGAAA3300.017.9993425
AACGATA8800.017.9993423
ATCGCGC9100.017.8905716
GCAACGA9000.017.84442921
GCAATCG9250.017.36152613
TCGCGAA3450.017.2167624
CAATCGC9750.016.69727114
GTCGGTA3700.016.05332443
TACGCAG3850.015.99926954
ACGATAA10300.015.59166124
CGATAAA11250.014.86167725
AGCGTCG4150.014.84296140
GTACCTG32800.014.5717471
Produced by FastQC (version 0.11.5)