FastQCFastQC Report
Wed 25 May 2016
SRR1699944_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699944_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5206408
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN182100.349761294159044No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN86100.16537313249365013No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN62650.12033248258684298No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN61080.11731696785960687No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN55930.10742531127026542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG57850.026.920841
TACCTGG57150.026.5223582
ACCTGGG64450.023.1086653
ACGATCG1705.456968E-1219.41134620
GTACACG5100.019.0044381
TACACGG4850.018.5973572
CCTGGGG50350.018.438354
ATCGCGA1954.9112714E-1116.9227123
TCGCGAA1954.9112714E-1116.9227124
CGCGAAA2007.2759576E-1116.49964325
ATAACGC3150.016.0629923
TATAACG2650.015.7730922
GTACATG135600.015.2213241
CTGTCGC8850.014.9147889
TAACGCA2950.014.9147884
ATGCGAT3900.014.66620811
TCGCGCA3600.014.66620817
TACATGG134550.014.6171582
AATCGCG3750.014.0795615
ACATGGG133850.014.0690423
Produced by FastQC (version 0.11.5)