FastQCFastQC Report
Wed 25 May 2016
SRR1699943_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699943_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4300019
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN203380.47297465429803914No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN96400.22418505592649707No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN76450.1777899120910861No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN60870.14155751404819372No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN46850.10895300695182975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN44970.10458093324703915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG51950.027.8993421
TACCTGG52150.027.5043332
ACCTGGG55700.025.1589263
CCTGGGG43650.020.9660664
ACGATCG755.4777578E-520.53287120
CGCGAAA902.2174112E-417.11072525
GTACACG3850.016.59171
ATCGCGA1206.4155684E-616.4996323
CTGGGGG25650.015.6096045
GTACCGG4450.015.3445891
TAGACCG1158.2277256E-515.3038265
TATACTG12100.014.7267715
GTACCCG4050.014.6846081
GTACATG104250.014.5155761
TACATGG102800.014.0598322
CGAACTA2204.9130904E-913.99952329
AATCGCG3000.013.93318215
TACACGG4300.013.8134832
TACGCTA1453.8415066E-513.6547085
ACATGGG103600.013.4840963
Produced by FastQC (version 0.11.5)