FastQCFastQC Report
Wed 25 May 2016
SRR1699942_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699942_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4623157
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG36400.021.9630071
TACCTGG36950.020.4810032
AATCGCG4950.019.11146415
ACCTGGG40450.018.3281483
AACGATA5400.017.9254823
CGCGCAA5400.017.92528718
TCGCGCA5550.017.83816717
GCGCAAC5400.017.51808219
CGCAACG5500.017.19957220
GTATCAA84100.015.9742481
ATCGCGC6000.015.76695716
GCAACGA6350.014.89726821
CAATCGC6750.014.66693814
GTACATG115050.014.4334871
TACATGG114350.014.1210532
GCAATCG6900.014.02924513
TCGCGTA950.006244825713.8942429
TATACTG11750.013.8548255
CCTGGGG36550.013.8435554
GGTATCA53900.013.6063611
Produced by FastQC (version 0.11.5)