FastQCFastQC Report
Wed 25 May 2016
SRR1699941_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699941_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4052972
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN335710.8283057469925773No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN105750.26091964119169836No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN99950.24660915496085342No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN85110.20999404881158812No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN76970.18991002158416095No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN66530.1641511463686401No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN64720.15968528773453158No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCN59070.14574490028551887No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN57590.1420932589714412No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGN49680.12257671654282339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN47920.11823422416932562No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAN46020.11354630626611781No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAN42790.10557684583066451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG36000.022.6366581
TACCTGG36250.022.0911642
ACCTGGG36850.021.2528083
TCGCGCA4350.020.73677617
CGCGCAA4550.019.82527218
AATCGCG4550.019.82331315
AACGATA4950.019.11051623
GCAATCG5000.018.91917613
GCAACGA4900.018.85818521
CGCAACG4850.018.59896520
CGACGTA951.59922E-518.525519
GCGCAAC5000.018.04099719
ACGATAA5050.017.8606124
ACGATCG902.216056E-417.11205720
GTACACG3500.016.9906021
TATATCG1451.251974E-716.688935
ATCGCGC5450.016.55116816
CGATAAA5500.016.39928825
CGTGCAA6600.015.66598610
CAATCGC6050.015.27206414
Produced by FastQC (version 0.11.5)