FastQCFastQC Report
Wed 25 May 2016
SRR1699940_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699940_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4718418
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN270140.5725224005164443No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN116990.24794327251210047No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCN94510.20030018535873678No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTN84470.1790218670749391No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATN62540.13254442484748066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN56900.12059126597092501No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTN54660.11584391209087452No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCN52560.1113932678283272No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCN48730.1032761404352052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGA1400.023.5705623
TCGCGCA6400.020.9690917
TACCTGG33200.020.6087672
GTACCTG35050.020.5564481
AATCGCG6100.020.55640215
AACGATA6500.019.29159523
CGCGAAA1504.674803E-1019.06576325
GCAACGA7150.018.46183821
CGCGCAA6800.018.44147518
GCGCAAC6800.018.44147519
CGCAACG7000.017.91457620
ATCGCGC7100.017.6620716
ACGATCG1751.9281288E-1017.60028520
ACGATAA7350.017.3597224
CGATAAA8550.017.23894925
ACCTGGG39450.016.6734813
GTACATG134400.015.9679081
GCAATCG7950.015.77283613
TACATGG133400.015.3210872
CAATCGC8250.015.19927914
Produced by FastQC (version 0.11.5)