FastQCFastQC Report
Wed 25 May 2016
SRR1699939_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699939_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799170
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN89190.23476180323596996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN71830.18906761213633502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN53990.14210998718141066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN38390.10104838688450371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG39900.025.4429911
TACCTGG39300.025.2458232
ACCTGGG43400.022.15123
ATAACGC2450.019.7543223
ATCGCGA1352.6047928E-919.55478323
CGCGCAA2600.018.6166118
GTACACG3650.018.0995831
CGCGAAA1355.5373675E-817.92521725
CCTGGGG35700.017.808824
TATAACG2850.017.7536852
ACGATCG1408.389725E-817.28503220
TCGCGCA2900.016.69075417
TAACGCA2750.015.9993674
AATCGCG3100.015.61393215
GCGCAAC3150.015.36609119
TACACGG3750.015.2527312
TATCACG2900.015.1718152
TCGCGAA1603.831392E-715.12440324
CGCAACG3400.014.88176420
GCAATCG3450.014.66763213
Produced by FastQC (version 0.11.5)