FastQCFastQC Report
Wed 25 May 2016
SRR1699938_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699938_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4211526
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN72910.1731201469491106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN49330.11713094018652621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG54300.029.055421
TACCTGG53450.028.976942
ACCTGGG59350.025.3163913
TATAACG3700.022.0002482
CCTGGGG45750.021.2055424
GGTATCA49650.020.592671
TAACGCA3850.019.9990394
GTATCAA90650.019.8594931
GTACCGG4350.019.7555581
ATAACGC4150.019.6135163
GTACACG4950.019.1415541
GTACCCG4550.016.9500351
CTGGGGG25750.016.9156915
AATCGCG2650.016.60297615
TACCCGG4400.016.0001812
TACACGG5100.015.9609652
CGCGCAA2800.015.71446418
ATCAACG113400.015.4807383
TCAACGC114350.015.40984254
CGCAACG3150.015.36525420
Produced by FastQC (version 0.11.5)