FastQCFastQC Report
Wed 25 May 2016
SRR1699937_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699937_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4491997
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN131070.2917855911301811No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTN49520.11024050105109154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG39850.025.5854191
TACCTGG39250.025.222722
ACCTGGG45300.021.5141683
AATCGCG4400.018.00025215
CGCGCAA4300.017.90722518
GTACCGG4100.017.7243331
ACGATCG1002.4858646E-517.59965720
TCGCGCA4850.016.33012417
GCAATCG5400.016.29634313
TAGACCG953.3496274E-416.210035
CGCAACG4900.016.1629520
CGCGAAA1259.454352E-615.83951525
GCGCAAC5050.015.68338719
AACGATA5050.015.68268823
CCTGGGG40150.015.67075354
GTACATG110750.015.1115731
GTACACG3800.015.0670971
CGATCGT2254.292815E-1014.66621841
GCAACGA5300.014.52785721
TACATGG110800.014.4944642
Produced by FastQC (version 0.11.5)