FastQCFastQC Report
Wed 25 May 2016
SRR1699936_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699936_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048063
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN74090.24307240368719413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN53850.176669576711505No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN46940.15399944161259135No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN40170.13178861460540678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN36880.12099487444977351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG47350.031.5939082
GTACCTG48400.031.399611
CGCGAAA454.072536E-529.33229425
ACCTGGG52500.027.9918483
TAACGCA3150.027.2371314
TATAACG3500.023.885262
CCTGGGG37100.023.1851634
ATAACGC3550.022.9291423
GTACACG3350.021.0390281
GTACCGG3050.020.2198871
TACACGG3700.020.2158322
CTGGGGG19200.019.4784765
TAAGTCG708.1291335E-418.8564745
TTTCGCG1002.4857798E-517.5993779
TAACGGC1154.278636E-617.21678236
GTACCCG3200.017.2072121
GTATCAA96100.016.5017381
GTATAAC6850.016.398351
CGATCGT1105.625875E-515.99995814
GGTATCA59650.015.4712051
Produced by FastQC (version 0.11.5)