FastQCFastQC Report
Wed 25 May 2016
SRR1699930_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699930_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5247950
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN144900.27610781352718683No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN130310.24830648157852112No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN96840.18452919711506396No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN95600.18216636972532133No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN87120.16600767918901665No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN85800.1634924113225164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN83520.15914785773492507No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN81510.1553177907563906No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN75670.14418963595308645No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN75080.1430653874369992No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN74290.14156003772901798No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN71310.13588162996979772No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN71300.135862574910203No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN71220.13571013443344546No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAGN69840.133080536209377No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN67740.12907897369449023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN66930.12753551386731962No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAN62180.11848436055983765No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN61800.11776026829523908No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCAN60400.11509255995198124No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTN59920.1141779170914357No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN55600.1059461313465258No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN54960.10472660753246506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG66250.031.9248471
TACCTGG69650.030.0999032
ACCTGGG71500.028.9211223
CCTGGGG51800.028.6238214
CTGGGGG35750.028.3675825
TAACGCA4050.027.7020974
TCGCGAA16900.022.26097124
CGCGAAA17250.021.9368425
CGACAAC6100.021.6391236
GGTATCA69700.021.4941161
ATCGCGA17550.021.43649123
CAACGGT6350.021.13323639
AGCGTCG17450.020.8016840
GTCGGTA17600.020.748643
CGTCGGT17500.020.74165342
ACGACCG755.479551E-520.532145
GTATCAA133100.020.4093171
CGAAATG18850.020.1915427
GTACCCG4750.019.9442791
TCGGTAG18650.019.5802644
Produced by FastQC (version 0.11.5)