FastQCFastQC Report
Wed 25 May 2016
SRR1699927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5303054
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN137710.25968055388461064No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN110260.2079179280467444No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN94900.1789534860478509No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN89950.16961924204430126No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN88670.1672055385443935No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN85960.16209527566568246No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN82580.15572158986123844No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN79600.15010218640051562No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN77550.14623648938894457No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN76670.14457706823275795No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN73970.13948566241263996No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN72170.13609139186589464No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN69910.13182969662386992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN68820.1297742772372297No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN68010.12824685549119433No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN64940.12245773850313423No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN64270.12119431557740125No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN62920.11864861266734225No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCAN55610.10486410283583761No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN55550.10475096048427944No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAGN54330.10245039933592982No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN54020.10186583051954591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG63000.030.4932821
TACCTGG68200.027.9983542
ACCTGGG68900.027.2669033
CTGGGGG38200.026.3178255
CCTGGGG53100.025.9758764
TAACGCA4700.024.3389994
GGTATCA63100.024.2651881
GCGTAAT2000.023.132231
GTATCAA125500.021.556731
CGACAAC6400.019.9372736
TCGCGAA14750.018.3460724
CGCGAAA15100.018.06652625
ATCGCGA15050.017.98036623
GTCGGTA14700.017.50917643
AATCGCG34550.017.3188115
CGCAACG35150.017.27417620
GCGCAAC35350.017.23786419
CGCGCAA34650.017.20517518
TCGCGCA34550.017.19146517
CAACGGT7450.017.1273239
Produced by FastQC (version 0.11.5)