FastQCFastQC Report
Wed 25 May 2016
SRR1699922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733642
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN113480.30393915645902847No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN79840.21383946291583392No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN76690.20540266045860853No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN72960.19541241500925904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN72700.19471604401278966No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN64020.17146796613065743No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN63710.17063767763486698No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN63000.16873604914450824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN54780.14672001225612955No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN53280.14270248727649837No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN52130.1396223847921145No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN51130.13694403480569375No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN50850.13619409680949593No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN50780.13600661231044647No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN47580.12743589235390002No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN43680.11699032740685904No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN42150.11289245192763528No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN41460.11104439043700494No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN40910.10957129794447351No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN37670.10089344398847024No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCAN37570.10062560898982816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG50250.032.3178181
ACCTGGG52200.030.764713
TACCTGG54100.030.0908832
CCTGGGG41750.029.7182394
AACGCGT901.6370905E-1129.33222633
CTGGGGG32000.027.017375
TAACGCA3350.024.9539474
GGTATCA51450.024.6259561
ACGCGTG1102.0190782E-1023.99909634
GTACCGG2450.022.4845681
GTACACG4000.022.0348761
CGCGAAA5750.021.42585425
CAACGGT6200.021.28951839
TACACGG3850.021.1417772
GTATCAA99100.021.0787721
ATCGCGA6100.020.91780323
TACCCGG4000.020.8989322
TCGCGAA6150.020.3900224
CGACAAC6500.020.30719836
GTCGGTA6600.019.99897643
Produced by FastQC (version 0.11.5)