FastQCFastQC Report
Wed 25 May 2016
SRR1699921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4002250
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN125120.31262414891623463No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN93830.2344431257417703No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN80150.20026235242675997No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN78300.19563995252670374No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN75500.18864388781310512No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN73490.18362171278655756No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN72650.18152289337247798No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN68610.17142857142857143No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN60530.15123992754075832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN59820.14946592541695294No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN59340.14826660003747894No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN58220.14546817415203947No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN58070.14509338497095384No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN56860.14207008557686301No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN52750.13180086201511648No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN51700.12917733774751702No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN48730.12175651196202136No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN48590.12140670872634142No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN47850.11955774876631896No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTN42410.1059653944656131No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCAN42250.10556561933912174No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN41180.10289212318071086No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAN40770.10186769941907678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG45350.032.4921071
ACCTGGG49500.029.15423
TACCTGG52150.028.4320432
CTGGGGG30050.026.7940945
CCTGGGG40400.026.4641134
GGTATCA49800.026.4484921
GTATCAA100750.021.796121
CGACAAC6800.021.0292236
TAACGCA3000.020.5323774
CAACGGT7100.020.45000839
ACGGTAA7350.020.35304841
ACAACGG7200.019.86043438
CGGTATA6000.019.43388626
TCGCGAA8650.019.33002924
GACAACG7450.019.19421837
ATCGCGA9050.018.96186423
CGCGAAA9050.018.71876325
GTACCGG3000.018.3547231
TACACGG2800.018.0705872
GTCGGTA9050.017.98811143
Produced by FastQC (version 0.11.5)