FastQCFastQC Report
Wed 25 May 2016
SRR1699918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4615860
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN138300.29961913922865946No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN111000.24047523105120172No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN97520.2112715723613801No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN90900.19692971623922736No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN77080.16698946675159124No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN73650.15955856546775682No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN70120.151911019831624No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN68720.14887799889944667No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN68470.14833638801870075No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN66680.14445845411255973No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN64930.1406671779473381No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN63000.13648594194797936No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN62020.13436282729545523No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN61430.1330846256168948No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN53120.11508147994089943No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN52060.1127850498065366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN51550.11168016360981485No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAN51190.11090024394154069No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN50420.10923208242884316No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN50310.10899377364131495No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN46960.10173618783931922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG65000.033.4878771
ACCTGGG67800.031.1811943
TACCTGG69700.030.836532
CCTGGGG53500.029.4005874
CTGGGGG40800.028.5232665
CGCGAAA8450.024.2127525
TCGCGAA8800.022.99974424
GGTATCA53100.022.985781
ATCGCGA8900.022.49413323
CGTCGGT9300.021.76225742
GTACACG3750.021.7377451
GTACCGG3250.021.692551
GTATCAA101100.021.4213281
TAACGCA3800.021.420124
GTCGGTA9200.021.28145243
AGCGTCG9450.021.18426140
GATCGCG9600.020.85393522
AACGCGT956.877108E-720.84142333
TACACGG3750.020.5324382
CGAAATG10200.020.05816727
Produced by FastQC (version 0.11.5)