FastQCFastQC Report
Wed 25 May 2016
SRR1699917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4966547
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN170050.34239079988571536No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN159350.3208466566409217No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN106980.21540116302131038No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN102370.2061190601840675No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN96120.1935348643635105No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN88370.1779304615460198No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN84550.17023900106049536No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN83210.16754094947656792No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN79910.16089649408331383No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN79800.160675012236872No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN78130.15731251511361918No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN76230.15348691958416985No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAN75890.15280233933153156No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAGN75200.15141304411294207No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTN74050.14909755208195957No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN68940.13880871357907215No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN67710.13633214384158654No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN62110.12505670438636743No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCAN61900.12463387540679671No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN58530.11784847702035237No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGN57410.11559338912930855No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN55450.11164698531998186No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACN53190.10709654011126846No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN52940.10659317227844617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG63950.031.2899671
ACCTGGG66200.029.275423
TACCTGG68400.028.9448722
CTGGGGG37400.028.6445875
CCTGGGG52200.028.0244034
TAACGCA2450.024.2428054
TCGCGAA16550.022.99800524
CGCGAAA16600.022.92873425
ATCGCGA16700.022.79143523
AGCGTCG16800.021.99943240
GATCGCG17450.021.68578122
CGTCGGT17200.021.6148542
CGAAATG18300.021.03918827
GTACACG3800.020.877511
GTCGGTA17800.020.39129443
CATCGGA9000.020.28713817
AAACGGT8900.020.27056923
TCGGTAG17850.020.21093844
TCGCGCA41100.020.12478417
GTACCGG3400.020.0928971
Produced by FastQC (version 0.11.5)