FastQCFastQC Report
Wed 25 May 2016
SRR1699916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4141221
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN101990.24628002224464715No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN80820.19515983329554254No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN78080.18854342716797776No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN76070.183689786176589No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN68130.1645166968872224No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN61620.1487966954673513No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN61380.1482171562444989No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN59740.14425697155500758No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN56770.13708517367220924No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN56420.1362400123055495No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN52700.12725715435133744No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN52340.12638784551705887No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN52170.1259773385675384No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN49490.11950581724568674No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTN46840.11310673832669157No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN45900.11083687637051971No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN45690.11032977955052388No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN44960.10856701441434784No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN44160.10663521700483988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG55650.031.8648221
TACCTGG57000.030.6821232
ACCTGGG58300.029.3564913
CCTGGGG46550.027.6930664
CTGGGGG32650.026.2769245
TAACGCA3000.024.1983554
CGCGAAA10450.020.8424525
ATCGCGA10650.020.65761823
TCGCGAA10650.020.4510424
GTACCGG3500.019.5107731
CGACAAC5650.019.0799636
AGCGTCG10850.019.06045240
GCGCGGA6800.018.76410729
CGTCGGT11250.018.57696242
GTACACG3450.018.5165351
GTCGGTA11050.018.51480143
CGAAATG11900.018.48792327
TCGTTCG600.00741384218.33385827
GGTATCA74250.018.156651
CGCATTT7150.018.15436641
Produced by FastQC (version 0.11.5)