FastQCFastQC Report
Wed 25 May 2016
SRR1699911_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699911_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4234064
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN130170.3074351261577529No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN89480.211333602893107No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN87220.20599594148789438No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN85140.20108340355743326No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN78850.1862276999119522No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN74330.17555237710152705No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN72180.17047451337532923No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN63680.15039923817873324No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN63480.14992687876234273No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN61610.14551031821909163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN61460.14515604865679876No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN60670.14329022896205632No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN55590.13129229978573775No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN53380.12607272823462282No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN50640.11960140423007304No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN50530.11934160655105827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN49490.11688533758582771No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN48540.11464163035797285No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN47860.11303560834224517No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTN46340.10944567677767741No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCAN46290.1093275869235798No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN44950.10616277883376349No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGN43990.10389545363508913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG56500.031.3286481
TACCTGG59350.029.430422
ACCTGGG58650.029.3690833
CCTGGGG45300.028.3603344
CTGGGGG33350.028.0343045
GGTATCA54450.024.9782411
GTACACG2650.021.6272871
TAACGCA2850.021.6127414
GTATCAA104800.021.2228851
CGCGTTA654.9369584E-420.30647730
AACGCGT1001.1251886E-619.79928233
CGACAAC6700.019.7010136
GTCGGTA7400.019.0258943
CAACGGT7000.018.85668239
AGCGTCG7300.018.68403440
TCGCGAA7700.018.57075524
ACGGTAA7350.018.55693241
CGCGAAA7600.018.52564425
CGCGCAA27500.018.47889318
GCGCAAC27550.018.44535619
Produced by FastQC (version 0.11.5)