FastQCFastQC Report
Wed 25 May 2016
SRR1699909_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699909_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3257115
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN95610.2935419842406547No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN70200.21552815912241355No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN69410.2131026997818622No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN63120.19379113110835816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN58120.17844012262385578No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN55620.1707646183816046No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN54370.16692686626047898No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN50870.1561811603213273No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN49490.15194428197960466No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN45870.14083015183682493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN44960.13803626829264548No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN44670.13714590980054434No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN44300.13600993517269117No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN43230.13272481935700767No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN39280.12059752265425078No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN37760.11593081607496204No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN37460.1150097555658919No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN37030.11368956883622469No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN36050.11068077117326224No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN33510.102882458863135No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCAN33310.10226841852375491No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTN33110.10165437818437481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG48700.032.9270441
TACCTGG51950.030.6165352
ACCTGGG51550.030.3420013
CCTGGGG38750.029.9758954
CTGGGGG26450.029.6928945
TAACGCA3450.026.1441614
TCGCGAA5550.025.76540824
CGCGAAA5750.024.86921925
ATCGCGA5950.024.77276423
GGTATCA43450.024.536121
CGTCGGT6300.023.04658942
AGCGTCG6200.022.70866440
GTCGGTA6200.022.70866443
GATCGCG6650.022.49592622
TACACGG2650.022.4140932
CGAAATG7100.020.45010627
GAACGAT7350.020.05352618
GTATCAA80450.020.0143571
TCGGTAG7050.019.9707444
TACCCGG3100.019.870082
Produced by FastQC (version 0.11.5)