FastQCFastQC Report
Wed 25 May 2016
SRR1699907_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699907_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4232124
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN126710.2994004901557705No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN100740.2380365036563201No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN84310.19921438974850456No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN82200.1942287135254071No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN71380.1686623548837416No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN64390.15214582559490222No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN63170.14926311232846676No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN62390.1474200661417293No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN60780.1436158297819251No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN60220.1422926171350367No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN55300.1306672488802313No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN55160.1303364457185092No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN52140.12320054894421809No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN51050.12062501004223884No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN45790.10819626268039405No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN44190.10441565511785571No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN43860.10363590480808219No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN43460.1026907529174476No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN43010.10162745704048368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG51800.033.4277151
ACCTGGG56750.029.8872683
TACCTGG57150.029.8709012
CTGGGGG32300.028.7420625
CCTGGGG46250.028.2066154
GTACACG3200.021.3415321
CGTCGGT7050.020.906642
GTACCGG2650.020.782991
GTCGGTA7000.020.74166543
TCGCGAA6900.020.72589524
GGTATCA51000.020.6476971
CGCGAAA6850.020.55599225
TCGGTAG7200.020.47104644
TAACGCA2700.020.3696824
AGCGTCG7150.020.30652640
ATCGCGA7200.020.1678923
AACGCGT1201.4350007E-820.16550633
CGCGCAA25650.020.06902318
AATCGCG25600.020.02228715
CGACAAC5800.019.72300536
Produced by FastQC (version 0.11.5)