FastQCFastQC Report
Wed 25 May 2016
SRR1699906_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699906_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4284568
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN133620.31186341306754845No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN127580.29776630922884173No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN100760.23516956668676983No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN83790.19556230639821798No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN72940.17023886655550804No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN70480.164497330886101No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN66810.15593170653377422No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN65710.15336435318566538No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN64560.15068030195809706No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN63680.14862641927960998No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN62790.146549197025231No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN59890.13978072001658043No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAGN55770.13016481474911823No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN55410.12932459001700988No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTN55270.1289978359545233No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAN54930.12820429037419875No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN53680.12528684338771146No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN47740.11142313530792369No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCAN46450.10841233001786879No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN45450.10607837242867892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG65150.033.7918931
TACCTGG69150.031.5576652
ACCTGGG70950.030.0129223
CCTGGGG57350.028.8833074
CTGGGGG40450.028.6057015
TAACGCA3050.027.4074824
GTACCGG3950.023.9901431
TCGCGAA11850.023.5790624
ATCGCGA12050.023.55286623
CGCGAAA12100.022.91006525
GTACCCG4150.022.833991
TACCCGG4250.022.256912
GTCGGTA12150.022.08863643
AGCGTCG12200.021.99990740
GTACACG4250.021.7781931
CGTCGGT12700.021.13204242
TCGGTAG12700.021.13204244
GATCGCG13600.020.86853222
CGAAATG13700.020.07384527
AACGATA32950.019.76411623
Produced by FastQC (version 0.11.5)