FastQCFastQC Report
Wed 25 May 2016
SRR1699904_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699904_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6587159
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN137120.20816257813117914No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN124250.18862456485413515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN111590.16940535365853474No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN101250.15370814641031133No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN96800.1469525784940063No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN93580.14206427991187096No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN91500.1389066212004295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN90850.13791985285310404No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN90030.13667500663032423No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN77750.11803267539162178No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN76560.11622613026344134No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN76480.11610468185146283No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN76190.11566443135804069No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN75600.11476874931969913No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN74070.11244604844060997No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN69840.10602446365724587No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN68540.10405092696259496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG69200.031.428421
TACCTGG72850.029.5950072
ACCTGGG73150.028.7518313
CTGGGGG38600.027.6998545
CCTGGGG55400.026.7652974
TAACGCA6100.022.7212724
GGTATCA84550.022.3085671
GTACCGG4400.021.5341281
CGACAAC8000.020.62527836
GTATCAA161200.020.1758961
CGTACGA550.004485737519.9999682
CAACGGT8450.019.78649739
CGCGAAA14450.019.03038825
TCGCGAA14500.018.96476624
ATCGCGA15050.018.41787523
GATCGCG15200.018.23611822
AGCGTCG15100.017.91995440
CCGACAA9350.017.88259335
ACGGTAA9250.017.83645241
ACCGGGG3600.017.7221933
Produced by FastQC (version 0.11.5)