FastQCFastQC Report
Wed 25 May 2016
SRR1699903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3900467
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN139110.35664960118878075No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN100750.25830240327632564No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN89400.22920332360201998No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN89330.2290238579123987No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN79030.20261676358241204No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN76820.196950775381512No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN76210.19538686008624095No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN66000.16921050735719595No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN64880.16633905632325566No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN63360.1624420870629081No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN62440.16008339514217143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN59860.1534688025818447No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN58560.15013586834602113No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN58480.14993076470073968No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGN56120.14388020716493694No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN53260.13654775184612508No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN51030.13083048773390468No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN50040.12829233012354674No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN48460.12424153312923811No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCAN46770.11990871862266748No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN45690.11713981941136793No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAN45290.11611430118496067No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTN45280.11608866322930049No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCN44450.11396071290950545No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGN43300.1110123480085846No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAN41920.1074743101274796No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCAN40720.10439775544825786No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGN39590.10150066645865738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG49350.031.3522151
ACCTGGG51850.029.4448833
TACCTGG53900.028.4474352
CTGGGGG35050.027.6789555
CCTGGGG45750.027.3602664
TAGACCG551.5950913E-423.9987015
CGCGAAA8200.023.87736525
TCGCGAA8250.023.73265624
GATCGCG8650.022.63519322
ATCGCGA8900.021.99937223
TAACGCA2750.021.598834
GTACCGG2950.020.9196451
CGACAAC7150.020.61479636
TCGACTA654.8742857E-420.344931
CAACGGT7250.020.33019339
GGTATCA54900.020.2739011
GTCGGTA9550.020.040843
GTATCAA100800.019.9849911
AGCGTCG9800.019.75428640
CGTCGGT9750.019.62995742
Produced by FastQC (version 0.11.5)