FastQCFastQC Report
Wed 25 May 2016
SRR1699895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6514071
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN145930.22402273478443818No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN116170.17833701843286634No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN112510.17271841218801576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN110180.1691415399064579No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN99020.15200939627461843No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN98090.15058171763863182No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN96120.1475574951516494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN87470.13427854869865558No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN86620.13297368112813016No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN84970.13044070290299262No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN83790.1286292396874397No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN79360.12182857693752494No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN77550.11904997658146496No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN76120.11685472878634574No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN75050.115212130785802No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN74470.11432175056120819No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN70910.10885665814818414No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN65190.1000756669677073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG75050.031.0914521
ACCTGGG79400.028.7597643
TACCTGG80850.028.5977022
CCTGGGG62900.027.8051284
CTGGGGG46600.027.0978245
GGTATCA76750.024.7471141
CAACGGT9150.021.87889539
CGACAAC9200.021.7609936
TAACGCA5000.021.5592924
ACAACGG9600.020.85412238
ACGGTAA9600.020.85252441
GTATCAA149000.020.8362921
CGCGAAA11950.020.80298225
TCGCGAA12050.020.44777524
ATCGCGA12100.020.18146523
GACAACG10250.019.74645237
AGCGTCG12200.019.6547940
CGTCGGT12400.019.51459142
GTCGGTA12550.019.10606443
CCGACAA10850.018.65448235
Produced by FastQC (version 0.11.5)