FastQCFastQC Report
Wed 25 May 2016
SRR1699894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6036766
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN133700.22147620099901172No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN114370.18945574501314114No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN114230.18922383276078614No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN101660.1684014255314849No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN99760.1652540449638101No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN98200.16266987986614023No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN84890.14062165073153407No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN84320.1396774365612316No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN83080.13762335661180175No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN81600.1351717128011919No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN81020.13421093347000695No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN80060.13262067802528704No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN71890.11908694158428536No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN71250.11802677128780542No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGCN70620.11698316615220798No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTN69220.11466404362865813No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN64050.10609985545240615No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCN63230.10474151226004122No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN63100.10452616516856875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG64850.030.0962541
TACCTGG68850.028.182132
ACCTGGG68400.027.8850983
CCTGGGG53100.026.5566144
CGCGTAA508.348611E-526.39918110
CTGGGGG39450.026.0986985
GGTATCA74150.025.638251
CGCGAAA12200.022.72098225
AACGCGT1105.2623363E-922.0004133
TCGCGAA12900.021.99968124
CGTCGGT12850.021.9128142
GTCGGTA13200.021.33178743
ATCGCGA13000.021.3227723
CGACAAC9350.021.17686536
ACAACGG9900.021.11150638
GTATCAA143800.020.8491331
GTACACG3600.020.8048781
AGCGTCG13500.020.69582440
CAACGGT10050.020.57715839
ACGGTAA10100.020.47376441
Produced by FastQC (version 0.11.5)