FastQCFastQC Report
Wed 25 May 2016
SRR1699891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2870795
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN72490.25250845149165996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN56990.19851643882617878No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN52630.18332900816672734No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN49340.171868768059022No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN49020.170754094249154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN45150.15727350786106287No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN43220.1505506314452965No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN41140.14330525168115452No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN38350.13358668940136792No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN35560.1238681271215813No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN35250.12278828686827167No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN34780.12115110971002807No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN33900.11808575673289107No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGN31290.1089941984711552No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCAN29060.10122631535863759No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAN29060.10122631535863759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG38400.032.30221
TACCTGG40900.030.0671632
ACCTGGG42300.028.8119963
CTGGGGG23450.027.6747135
CCTGGGG33600.027.3678654
GGTATCA44550.021.3644141
CGACAAC3450.021.04293636
GTATCAA79750.019.8633791
TAACGCA3000.019.7991474
GTCTCGC4250.019.6992451
GTACACG3150.019.5840451
GCGTAAT1504.5656634E-1019.0944461
TCGCTCT4400.018.999184
CAACGGT3900.018.61490639
CGCTCTG4050.018.468345
TACACGG3100.018.4508172
ACAACGG4200.017.80906538
CTCGCTC4450.017.7969863
TAGCGTT6600.017.66590516
CGATCGA4000.017.59985427
Produced by FastQC (version 0.11.5)