FastQCFastQC Report
Wed 25 May 2016
SRR1699890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1699890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516859
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN83140.23640413221002035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN69010.19622623483056897No Hit
GTACCTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTN62560.17788600566585128No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAN56980.16201957485358384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTN54000.15354610463484603No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGN53890.1532333255328121No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGN48060.13665603312501298No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATN45030.12804039058716882No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGN44040.12522537866886332No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAAN39460.11220239423872268No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGN37880.10770974895496237No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATN37060.10537812292161841No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTAN36780.10458195793462291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG46050.031.6825521
TACCTGG47950.030.09652
ACCTGGG49400.028.3669913
CCTGGGG37750.027.1562464
CTGGGGG26400.026.8319825
GGTATCA44650.024.531651
TAACGCA3250.024.3680024
CCGTTCG551.5946997E-423.99947432
GTACCGG2350.023.4457421
GTATCAA89100.021.0990641
CGCGTAT654.9354695E-420.30724735
TACACGG2600.020.306672
TACCCGG2300.020.0859452
GTACACG2900.019.7591021
CGACAAC3250.019.63033936
CGTGACG808.998273E-519.2490319
TATCACG2400.019.2490272
GTCGGTA4400.018.99904343
GTCTCGC5150.018.4014951
CGCTCTG4800.018.3324075
Produced by FastQC (version 0.11.5)